Coding

Part:BBa_K2022001:Design

Designed by: Gavin Sutton, Shivani Shah   Group: iGEM16_UNSW_Australia   (2016-10-10)


Attachment Protein g3p, N-terminal Domain, His-tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

See: https://parts.igem.org/wiki/index.php?title=Part:BBa_K2022000

The his-tag was created by adding six histidine residues just prior to double stop codons that stop translation. The DNA sequence for these six His residues is catcaccatcaccatcac, which is (roughly) E. coli codon optimised by balancing between the two codons for His


Source

See: https://parts.igem.org/wiki/index.php?title=Part:BBa_K2022000

References